pkh26 red fluorescent membrane staining dye Search Results


pkh26  (MedChemExpress)
95
MedChemExpress pkh26
Pkh26, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent membrane dye pkh26  (Millipore)
90
Millipore fluorescent membrane dye pkh26
Fluorescent Membrane Dye Pkh26, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pkh26 red fluorescent cell linker kit  (Millipore)
90
Millipore pkh26 red fluorescent cell linker kit
Pkh26 Red Fluorescent Cell Linker Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pkh26 red fluorescent cell linker kit  (Merck & Co)
90
Merck & Co pkh26 red fluorescent cell linker kit
Pkh26 Red Fluorescent Cell Linker Kit, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pkh26 red fluorescent labeling kit  (Thermo Fisher)
90
Thermo Fisher pkh26 red fluorescent labeling kit
Pkh26 Red Fluorescent Labeling Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pkh26 fluorescent dye  (Beijing Solarbio Science)
90
Beijing Solarbio Science pkh26 fluorescent dye
Macrophage polarisation assays following treatment with exosomes from HPV E6 + CESC. ( A ) Establishment of HPV E6 + C33A cell lines. ( B ) Identification of exosomes via TEM and NTA analyses. ( C ) Immunofluorescence staining showing the internalisation of <t>PKH26-labelled</t> exosomes (red) derived from HPV E6 + C33A cells by macrophages (blue). ( D ) Expression levels of TNFA and ARG1 mRNA in macrophages after treatment with exosomes from HPV E6 + C33A cells. ( E , F , H , I ) IHC staining for iNOS and CD163 in macrophages following treatment with exosomes from either HPV E6 + C33A cells or GW4869 pre-treated cell-derived exosomes. ( G , J ) Cytokine levels in the culture medium of macrophages treated with exosomes from either HPV E6 + C33A cells or GW4869 pre-treated cell-derived exosomes.
Pkh26 Fluorescent Dye, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pkh26 fluorescent dye/product/Beijing Solarbio Science
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pkh26 red fluorescent cell linker mini kit cat#: mini26-1kt  (Merck KGaA)
90
Merck KGaA pkh26 red fluorescent cell linker mini kit cat#: mini26-1kt
Macrophage polarisation assays following treatment with exosomes from HPV E6 + CESC. ( A ) Establishment of HPV E6 + C33A cell lines. ( B ) Identification of exosomes via TEM and NTA analyses. ( C ) Immunofluorescence staining showing the internalisation of <t>PKH26-labelled</t> exosomes (red) derived from HPV E6 + C33A cells by macrophages (blue). ( D ) Expression levels of TNFA and ARG1 mRNA in macrophages after treatment with exosomes from HPV E6 + C33A cells. ( E , F , H , I ) IHC staining for iNOS and CD163 in macrophages following treatment with exosomes from either HPV E6 + C33A cells or GW4869 pre-treated cell-derived exosomes. ( G , J ) Cytokine levels in the culture medium of macrophages treated with exosomes from either HPV E6 + C33A cells or GW4869 pre-treated cell-derived exosomes.
Pkh26 Red Fluorescent Cell Linker Mini Kit Cat#: Mini26 1kt, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pkh26 fluorescent probe 40780es20  (Yeasen Biotechnology)
90
Yeasen Biotechnology pkh26 fluorescent probe 40780es20
Role of EMs exosomes on macrophage polarization. (A) The exosomes were labeled with a <t>PKH26</t> fluorescent probe and then co‐cultured with macrophages induced from THP‐1 cells, and the uptake of exosomes by macrophages was observed using a fluorescence microscope (scale bar: 200 μm). (B) RT‐qPCR was performed to detect the mRNA expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (C) Western blot was used to detect the protein expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (D) The proportion of CD86+ (M1) and CD206+ (M2) macrophages was detected using a flow cytometer. Data represent mean ± SD from three independent experiments. **: P < 0.01, ***: P < 0.001, compared with control exosomes.
Pkh26 Fluorescent Probe 40780es20, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pkh26 red fluorescent cell connection kit  (Tanon Science & Technology)
90
Tanon Science & Technology pkh26 red fluorescent cell connection kit
Role of EMs exosomes on macrophage polarization. (A) The exosomes were labeled with a <t>PKH26</t> fluorescent probe and then co‐cultured with macrophages induced from THP‐1 cells, and the uptake of exosomes by macrophages was observed using a fluorescence microscope (scale bar: 200 μm). (B) RT‐qPCR was performed to detect the mRNA expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (C) Western blot was used to detect the protein expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (D) The proportion of CD86+ (M1) and CD206+ (M2) macrophages was detected using a flow cytometer. Data represent mean ± SD from three independent experiments. **: P < 0.01, ***: P < 0.001, compared with control exosomes.
Pkh26 Red Fluorescent Cell Connection Kit, supplied by Tanon Science & Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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red fluorescent membrane dye pkh26  (PTI Research Inc)
90
PTI Research Inc red fluorescent membrane dye pkh26
Role of EMs exosomes on macrophage polarization. (A) The exosomes were labeled with a <t>PKH26</t> fluorescent probe and then co‐cultured with macrophages induced from THP‐1 cells, and the uptake of exosomes by macrophages was observed using a fluorescence microscope (scale bar: 200 μm). (B) RT‐qPCR was performed to detect the mRNA expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (C) Western blot was used to detect the protein expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (D) The proportion of CD86+ (M1) and CD206+ (M2) macrophages was detected using a flow cytometer. Data represent mean ± SD from three independent experiments. **: P < 0.01, ***: P < 0.001, compared with control exosomes.
Red Fluorescent Membrane Dye Pkh26, supplied by PTI Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pkh26 fluorescent staining kit  (Zynaxis Inc)
90
Zynaxis Inc pkh26 fluorescent staining kit
Role of EMs exosomes on macrophage polarization. (A) The exosomes were labeled with a <t>PKH26</t> fluorescent probe and then co‐cultured with macrophages induced from THP‐1 cells, and the uptake of exosomes by macrophages was observed using a fluorescence microscope (scale bar: 200 μm). (B) RT‐qPCR was performed to detect the mRNA expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (C) Western blot was used to detect the protein expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (D) The proportion of CD86+ (M1) and CD206+ (M2) macrophages was detected using a flow cytometer. Data represent mean ± SD from three independent experiments. **: P < 0.01, ***: P < 0.001, compared with control exosomes.
Pkh26 Fluorescent Staining Kit, supplied by Zynaxis Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pkh26 fluorescent staining kit/product/Zynaxis Inc
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pkh26 fluorescent staining kit - by Bioz Stars, 2026-03
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ez c1 3 80 software  (Nikon)
99
Nikon ez c1 3 80 software
Role of EMs exosomes on macrophage polarization. (A) The exosomes were labeled with a <t>PKH26</t> fluorescent probe and then co‐cultured with macrophages induced from THP‐1 cells, and the uptake of exosomes by macrophages was observed using a fluorescence microscope (scale bar: 200 μm). (B) RT‐qPCR was performed to detect the mRNA expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (C) Western blot was used to detect the protein expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (D) The proportion of CD86+ (M1) and CD206+ (M2) macrophages was detected using a flow cytometer. Data represent mean ± SD from three independent experiments. **: P < 0.01, ***: P < 0.001, compared with control exosomes.
Ez C1 3 80 Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Macrophage polarisation assays following treatment with exosomes from HPV E6 + CESC. ( A ) Establishment of HPV E6 + C33A cell lines. ( B ) Identification of exosomes via TEM and NTA analyses. ( C ) Immunofluorescence staining showing the internalisation of PKH26-labelled exosomes (red) derived from HPV E6 + C33A cells by macrophages (blue). ( D ) Expression levels of TNFA and ARG1 mRNA in macrophages after treatment with exosomes from HPV E6 + C33A cells. ( E , F , H , I ) IHC staining for iNOS and CD163 in macrophages following treatment with exosomes from either HPV E6 + C33A cells or GW4869 pre-treated cell-derived exosomes. ( G , J ) Cytokine levels in the culture medium of macrophages treated with exosomes from either HPV E6 + C33A cells or GW4869 pre-treated cell-derived exosomes.

Journal: Scientific Reports

Article Title: HPV16 E6-induced M2 macrophage polarization in the cervical microenvironment via exosomal miR-204-5p

doi: 10.1038/s41598-024-74399-0

Figure Lengend Snippet: Macrophage polarisation assays following treatment with exosomes from HPV E6 + CESC. ( A ) Establishment of HPV E6 + C33A cell lines. ( B ) Identification of exosomes via TEM and NTA analyses. ( C ) Immunofluorescence staining showing the internalisation of PKH26-labelled exosomes (red) derived from HPV E6 + C33A cells by macrophages (blue). ( D ) Expression levels of TNFA and ARG1 mRNA in macrophages after treatment with exosomes from HPV E6 + C33A cells. ( E , F , H , I ) IHC staining for iNOS and CD163 in macrophages following treatment with exosomes from either HPV E6 + C33A cells or GW4869 pre-treated cell-derived exosomes. ( G , J ) Cytokine levels in the culture medium of macrophages treated with exosomes from either HPV E6 + C33A cells or GW4869 pre-treated cell-derived exosomes.

Article Snippet: For labelling, exosomes were resuspended in Dilutant C and mixed with PKH26 fluorescent dye (Solarbio, China).

Techniques: Immunofluorescence, Staining, Derivative Assay, Expressing, Immunohistochemistry

Role of EMs exosomes on macrophage polarization. (A) The exosomes were labeled with a PKH26 fluorescent probe and then co‐cultured with macrophages induced from THP‐1 cells, and the uptake of exosomes by macrophages was observed using a fluorescence microscope (scale bar: 200 μm). (B) RT‐qPCR was performed to detect the mRNA expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (C) Western blot was used to detect the protein expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (D) The proportion of CD86+ (M1) and CD206+ (M2) macrophages was detected using a flow cytometer. Data represent mean ± SD from three independent experiments. **: P < 0.01, ***: P < 0.001, compared with control exosomes.

Journal: Journal of Cell Communication and Signaling

Article Title: Endometriosis‐derived exosomes encapsulated miR‐196a‐5p mediate macrophage polarization through regulation of the Hippo pathway

doi: 10.1002/ccs3.70020

Figure Lengend Snippet: Role of EMs exosomes on macrophage polarization. (A) The exosomes were labeled with a PKH26 fluorescent probe and then co‐cultured with macrophages induced from THP‐1 cells, and the uptake of exosomes by macrophages was observed using a fluorescence microscope (scale bar: 200 μm). (B) RT‐qPCR was performed to detect the mRNA expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (C) Western blot was used to detect the protein expression levels of macrophage polarization markers (iNOS and CD86 for M1 markers; CD206 and CD163 for M2 markers). (D) The proportion of CD86+ (M1) and CD206+ (M2) macrophages was detected using a flow cytometer. Data represent mean ± SD from three independent experiments. **: P < 0.01, ***: P < 0.001, compared with control exosomes.

Article Snippet: Exosomes were labeled with the PKH26 fluorescent probe (40780ES20, YEASEN, China) and co‐cultured (20 μg/mL) with THP‐1‐derived macrophages.

Techniques: Labeling, Cell Culture, Fluorescence, Microscopy, Quantitative RT-PCR, Expressing, Western Blot, Flow Cytometry, Control